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The Directional Freezing Effects on Biological Cells in a Microfluidic Cell Culture System

[+] Author Affiliations
Yuhui Li, Fen Wang, Yanyan Lu, Hao Wang

Peking University, Beijing, China

Paper No. MNHMT2009-18301, pp. 643-650; 8 pages
  • ASME 2009 Second International Conference on Micro/Nanoscale Heat and Mass Transfer
  • ASME 2009 Second International Conference on Micro/Nanoscale Heat and Mass Transfer, Volume 1
  • Shanghai, China, December 18–21, 2009
  • Conference Sponsors: Nanotechnology Institute
  • ISBN: 978-0-7918-4389-5 | eISBN: 978-0-7918-3864-8
  • Copyright © 2009 by ASME


Cryotherapy is a prospective also green method for malignant tumor treatment. At low temperature, the cell viability relates with the cooling rate, temperature threshold, freezing interface as well as ice formation. In this paper a series of directional freezing processes and cell responses in a culture microchip were experimentally investigated. The temperature in the microchip was manipulated by a thermoelectric cooler. The surviving cells, necrotic and apoptotic cells under different cryotreatment (duration of the freezing process, freeze-thaw cycle, post-culture et al) were stained and distinguished by PI and FITC-Annexin V. The locations of the ice front and cell death boundary were observed and recorded through an inverted microscopy. By controlling the cooling process in a microfluidic channel, it is possible to recreate a sketch of biological effect during the process of simulated cryosurgery.

Copyright © 2009 by ASME



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