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Dielectrophoresis Separation of Colon Cancer Cell

[+] Author Affiliations
Fang Yang, Xiaoming Yang, P. Wood, W. Hrushesky, G. R. Wang

University of South Carolina, Columbia, SC

H. Jiang

GraceFlow Technology, Irmo, SC

Paper No. MNHMT2009-18519, pp. 351-356; 6 pages
doi:10.1115/MNHMT2009-18519
From:
  • ASME 2009 Second International Conference on Micro/Nanoscale Heat and Mass Transfer
  • ASME 2009 Second International Conference on Micro/Nanoscale Heat and Mass Transfer, Volume 1
  • Shanghai, China, December 18–21, 2009
  • Conference Sponsors: Nanotechnology Institute
  • ISBN: 978-0-7918-4389-5 | eISBN: 978-0-7918-3864-8
  • Copyright © 2009 by ASME

abstract

Separation of cancer cells from the other biological cells is important for clinical cancer diagnosis and cancer treatment. In this presentation, we use conventional dielectrophoresis (c-DEP) in a microfluidic chip to manipulate and collect colorectal cancer HCT116 cell. It is noticed that at particular AC frequency band, the HCT116 cell are deflected to a side channel from a main channel clearly after the electric activation. This motion caused by negative DEP can be used to separate the cancer cell from others. In this manuscript, we report the chip design, flow condition, the DEP spectrum of the cancer cell, and the separation and collection efficiency as well.

Copyright © 2009 by ASME

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