0

Full Content is available to subscribers

Subscribe/Learn More  >

Quantitative DNA Hybridization Assay on a Multiplexed Surface Microfluidic Device

[+] Author Affiliations
Ravi Prakash, Dipankar Chugh, Karan V. I. S. Kaler

University of Calgary, Calgary, AB, Canada

Paper No. MNHMT2009-18270, pp. 103-111; 9 pages
doi:10.1115/MNHMT2009-18270
From:
  • ASME 2009 Second International Conference on Micro/Nanoscale Heat and Mass Transfer
  • ASME 2009 Second International Conference on Micro/Nanoscale Heat and Mass Transfer, Volume 1
  • Shanghai, China, December 18–21, 2009
  • Conference Sponsors: Nanotechnology Institute
  • ISBN: 978-0-7918-4389-5 | eISBN: 978-0-7918-3864-8
  • Copyright © 2009 by ASME

abstract

A Dielectrophoresis (DEP) based Surface Microfluidic (SMF) device has been successfully configured to facilitate multiplexed DNA hybridization assay. In this paper, we have demonstrated the utility of a 2×2 multiplexed DEP based droplet dispensing and conveyance schemes to manipulate sub-nanoliter DNA sample droplets and facilitate high throughput applications. To quantify and study various aspects of the multiplexed DNA hybridization assay, a photo multiplier tube (PMT) was utilized to optically monitor the individual droplets. Applications of the proposed assay scheme are envisioned in generalization towards an MxN matrix which can conduct numerous biochemical assays in a parallel, automated fashion, consuming minimal quantities of chemical and biochemical reagents and providing means for reliable and low cost bio-diagnostics.

Copyright © 2009 by ASME
Topics: Microfluidics , DNA

Figures

Tables

Interactive Graphics

Video

Country-Specific Mortality and Growth Failure in Infancy and Yound Children and Association With Material Stature

Use interactive graphics and maps to view and sort country-specific infant and early dhildhood mortality and growth failure data and their association with maternal

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging and repositioning the boxes below.

Related eBook Content
Topic Collections

Sorry! You do not have access to this content. For assistance or to subscribe, please contact us:

  • TELEPHONE: 1-800-843-2763 (Toll-free in the USA)
  • EMAIL: asmedigitalcollection@asme.org
Sign In