0

Full Content is available to subscribers

Subscribe/Learn More  >

Electrokinetic Mixing in Microplates and its Applications to Kinase and ELISA-Assay Type Reactions

[+] Author Affiliations
Maud-Alix Mader, Frederic Bottausci, Igor Mezic

University of California at Santa Barbara, Santa Barbara, CA

Paper No. FEDSM2009-78530, pp. 539-542; 4 pages
doi:10.1115/FEDSM2009-78530
From:
  • ASME 2009 Fluids Engineering Division Summer Meeting
  • Volume 2: Fora
  • Vail, Colorado, USA, August 2–6, 2009
  • Conference Sponsors: Fluids Engineering Division
  • ISBN: 978-0-7918-4373-4 | eISBN: 978-0-7918-3855-6
  • Copyright © 2009 by ASME

abstract

While 384 well-plate format is still commonly used by the Biomolecular community, miniaturized formats (1536 and 3456 well-plates) are gaining in popularity, due to promise of higher throughput and lower reagent consumption. However, decrease in reagent volume in such miniaturized formats leads to issues of accuracy and sensitivity caused by incomplete mixing and the mechanical stirring methods satisfactory used for 384 well-plate formats, give poor mixing with lower volumes. Here we present results obtained in 1536-well and 3456-well plates equipped with electrodes where we induced fluid flow by the action of electromagnetic fields. Improvement in two types of reactions are obtained, related to ELISA-type assays and kinase-based assays. For example, in the kinase reaction, we show that, when using the electrode-enabled mixing technology, an 8 minutes protocol in 1536-well plate format is equivalent in accuracy to a 90 minutes protocol in standard 96-well plate format mixed using an orbital shaker. This mixing technology is scalable, due to modern developments in electrode manufacturing and can be used in super-high throughput screening formats, such as 10000-well or droplets.

Copyright © 2009 by ASME

Figures

Tables

Interactive Graphics

Video

Country-Specific Mortality and Growth Failure in Infancy and Yound Children and Association With Material Stature

Use interactive graphics and maps to view and sort country-specific infant and early dhildhood mortality and growth failure data and their association with maternal

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging and repositioning the boxes below.

Related eBook Content
Topic Collections

Sorry! You do not have access to this content. For assistance or to subscribe, please contact us:

  • TELEPHONE: 1-800-843-2763 (Toll-free in the USA)
  • EMAIL: asmedigitalcollection@asme.org
Sign In