0

Full Content is available to subscribers

Subscribe/Learn More  >

Computational Analysis of a Microfluidic Magnetophoretic Device for DNA Isolation

[+] Author Affiliations
C. Hale, J. Darabi

Southern Illinois University Edwardsville, Edwardsville, IL

Paper No. FEDSM2018-83482, pp. V003T21A011; 7 pages
doi:10.1115/FEDSM2018-83482
From:
  • ASME 2018 5th Joint US-European Fluids Engineering Division Summer Meeting
  • Volume 3: Fluid Machinery; Erosion, Slurry, Sedimentation; Experimental, Multiscale, and Numerical Methods for Multiphase Flows; Gas-Liquid, Gas-Solid, and Liquid-Solid Flows; Performance of Multiphase Flow Systems; Micro/Nano-Fluidics
  • Montreal, Quebec, Canada, July 15–20, 2018
  • Conference Sponsors: Fluids Engineering Division
  • ISBN: 978-0-7918-5157-9
  • Copyright © 2018 by ASME

abstract

A continuous flow magnetophoretic DNA isolation device was modeled using COMSOL Multiphysics and MATLAB to study and optimize the fluidic flow and geometric parameters of the device. The optimization was performed with the intention of increasing the volumetric flow rate and thus increasing the sample processing capabilities of the device. Key features of the device include oppositely oriented magnets that provide extremely strong magnetic field gradients and improve the capture rate of the magnetic particles. Another feature of the device is a nickel grid arrayed on the bottom surface of the channel to create localized amplified magnetic field gradients, and to provide even spreading and capture of the particles on the bottom surface of the device. These features in conjunction with the optimization of the fluid flow and geometric parameters provide the ability for higher velocity sampling and increased throughput. The simulation was conducted in two steps. First the fluid flow and the magnetic field simulations were performed in COMSOL Multiphysics. Next, the values of velocity fields and magnetic field gradients throughout the computational domain were exported to MATLAB to calculate the motion and trajectory of the particles. The particle trajectory analysis was performed until the particle either reached the bottom of the channel or was not captured within the set length and flowed out to the outlet channel.

Copyright © 2018 by ASME
Topics: Microfluidics , DNA

Figures

Tables

Interactive Graphics

Video

Country-Specific Mortality and Growth Failure in Infancy and Yound Children and Association With Material Stature

Use interactive graphics and maps to view and sort country-specific infant and early dhildhood mortality and growth failure data and their association with maternal

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging and repositioning the boxes below.

Related eBook Content
Topic Collections

Sorry! You do not have access to this content. For assistance or to subscribe, please contact us:

  • TELEPHONE: 1-800-843-2763 (Toll-free in the USA)
  • EMAIL: asmedigitalcollection@asme.org
Sign In