0

Full Content is available to subscribers

Subscribe/Learn More  >

Cell Response to Cyclic Strain at Focal Adhesions

[+] Author Affiliations
Toshihiko Shiraishi, Tomohiro Fukuno

Yokohama National University, Yokohama, Japan

Paper No. IMECE2016-66843, pp. V003T04A086; 5 pages
doi:10.1115/IMECE2016-66843
From:
  • ASME 2016 International Mechanical Engineering Congress and Exposition
  • Volume 3: Biomedical and Biotechnology Engineering
  • Phoenix, Arizona, USA, November 11–17, 2016
  • Conference Sponsors: ASME
  • ISBN: 978-0-7918-5053-4
  • Copyright © 2016 by ASME

abstract

Cells are known to sense and respond to mechanical stimulations. The fact shows that there are some cellular mechanosensors for mechanical stimulations. One of the candidates of the mechanosensors is focal adhesions which are large macromolecular assemblies via which mechanical force and regulatory signals may be transmitted between the extracellular matrix and an interacting cell. Although it is quite important to clarify the mechanism of sensing and responding to the mechanical vibration via focal adhesions, there was no micro device applying time-varied mechanical loading to a single focal adhesion of the order of a micrometer. In order to solve the challenging issue, we developed a magnetic micropillar substrate which is able to apply cyclic strain to focal adhesions of a cell. Using the substrate, we investigated how a single osteoblast-like cell changed the direction of migration on micropillars cyclically deflected at 5 Hz and revealed the relationship between the cell migration and the traction force. The experimental results indicate that a cell may sense the cyclic strain and reduce the traction force which is not enough to move the cell body forward leading to changing the migration direction toward the place without cyclic strain.

Copyright © 2016 by ASME

Figures

Tables

Interactive Graphics

Video

Country-Specific Mortality and Growth Failure in Infancy and Yound Children and Association With Material Stature

Use interactive graphics and maps to view and sort country-specific infant and early dhildhood mortality and growth failure data and their association with maternal

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging and repositioning the boxes below.

Related eBook Content
Topic Collections

Sorry! You do not have access to this content. For assistance or to subscribe, please contact us:

  • TELEPHONE: 1-800-843-2763 (Toll-free in the USA)
  • EMAIL: asmedigitalcollection@asme.org
Sign In