Full Content is available to subscribers

Subscribe/Learn More  >

Development of IKVAV Modified PLLA Guide Tube Having Unidirectional Fibers on Inner Surface to Enhance Axonal Extension

[+] Author Affiliations
Y. Akizawa, Y. Morita, E. Nakamachi

Doshisha University, Kyoto, Japan

Y. Hsu, T. Yamaoka

National Cerebral and Cardiovascular Center Research Institute, Osaka, Japan

Paper No. IMECE2016-66458, pp. V003T04A073; 6 pages
  • ASME 2016 International Mechanical Engineering Congress and Exposition
  • Volume 3: Biomedical and Biotechnology Engineering
  • Phoenix, Arizona, USA, November 11–17, 2016
  • Conference Sponsors: ASME
  • ISBN: 978-0-7918-5053-4
  • Copyright © 2016 by ASME


The bi-layer poly-L-lactic acid (PLLA) guide tube for peripheral nerve regeneration was developed by electrospinning method to enhance axonal extension. The inner layer of the guide tube had unidirectional nanofiber and Ile-Lys-Val-Ala-Val (IKVAV) modified surface. IKVAV modified unidirectional fiber sheets which had different fiber diameters from 400 to 1200 nm were fabricated to optimize fiber diameter of the guide tube for axonal extension. Fiber sheets without the IKVAV were prepared as the control group. PC12 cells seeded on the fiber sheets were cultured for 6 days. Axons were most extended in fiber diameter of 1090 nm with IKVAV and 1160 nm without IKVAV. Maximum axon length increased by about 25% due to IKVAV modification. The bi-layer guide tube which had fiber diameter about 1133 nm was fabricated. It was confirmed that the inner layer had unidirectional fibers and the outer layer had random fibers in the guide tube. CS, which corresponds to radial compressive stiffness, of the guide tube and the sciatic nerve were 230 N/mm2 and 1.64 N/mm2 respectively. Since radial compressive stiffness of the guide tube was higher than that of the sciatic nerve, the developed guide tube was expected to enhance axonal extension without narrowing.

Copyright © 2016 by ASME
Topics: Fibers



Interactive Graphics


Country-Specific Mortality and Growth Failure in Infancy and Yound Children and Association With Material Stature

Use interactive graphics and maps to view and sort country-specific infant and early dhildhood mortality and growth failure data and their association with maternal

Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging and repositioning the boxes below.

Related eBook Content
Topic Collections

Sorry! You do not have access to this content. For assistance or to subscribe, please contact us:

  • TELEPHONE: 1-800-843-2763 (Toll-free in the USA)
  • EMAIL: asmedigitalcollection@asme.org
Sign In