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In Vitro Development of Microvessels Using a Scaffold of Cylindrical PLGA

[+] Author Affiliations
Yen-Ting Tung, Gou-Jen Wang

National Chung-Hsing University, Taichung, Taiwan

Paper No. DETC2016-59550, pp. V004T08A001; 7 pages
doi:10.1115/DETC2016-59550
From:
  • ASME 2016 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference
  • Volume 4: 21st Design for Manufacturing and the Life Cycle Conference; 10th International Conference on Micro- and Nanosystems
  • Charlotte, North Carolina, USA, August 21–24, 2016
  • Conference Sponsors: Design Engineering Division, Computers and Information in Engineering Division
  • ISBN: 978-0-7918-5014-5
  • Copyright © 2016 by ASME

abstract

The microvascular network is a simple but critical system that is responsible for various important biological mechanisms in the bodies of all animals. The ability to generate a functional microvessel in vitro not only makes it possible to engineer vital tissue of considerable size but also serves as a platform for biomedical studies. In this study, we propose a simple method for fabricating customized cylinder micro-scaffolds for the in vitro development of microvascular networks. By integrating micro-electro-mechanical systems techniques with thermal reflow, we design and fabricate a micro-scale hemi-cylinder photoresist template. Then, a replica mold of polydimethylsiloxane, produced by casting, is used to generate microvascular network scaffolds of poly(lactide-co-glycolide) (PLGA). We selected the human umbilical vein endothelial cell (HUVEC) as our model endothelial cell, seeded it onto both sides of the PLGA scaffold, and cultured it using a traditional approach with no pumping system. Results from fluorescent staining demonstrate that the scaffold was covered with HUVECs and that the desired microvascular network and pattern was generated in vitro. The proposed method enables the culture of cells on a scaffold using a conventional culture approach and allows continuous monitoring of cell conditions. The cell-covered scaffold can serve as a framework for building large tissues, while the formed microvascular network, after degradation of the biodegradable PLGA cylinder, can be used as the core of a vascular chip for in vitro circulation studies.

Copyright © 2016 by ASME
Topics: PLGA

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