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Effects of Cyclic Strain at Focal Adhesions on Migration of an Osteoblast

[+] Author Affiliations
Tomohiro Fukuno, Toshihiko Shiraishi

Yokohama National University, Hodogaya-ku, Japan

Paper No. IMECE2015-51447, pp. V003T03A102; 7 pages
doi:10.1115/IMECE2015-51447
From:
  • ASME 2015 International Mechanical Engineering Congress and Exposition
  • Volume 3: Biomedical and Biotechnology Engineering
  • Houston, Texas, USA, November 13–19, 2015
  • Conference Sponsors: ASME
  • ISBN: 978-0-7918-5738-0
  • Copyright © 2015 by ASME

abstract

Cells respond to not only biochemical signals but also mechanical forces, which indicates that cells have some mechanosensors that convert mechanical forces into biochemical signals. According to recent reports, one of the candidates of the mechanosensors is focal adhesions that form multi-protein structures having mechanical links between intracellular cytoskeletons and extracellular matrices. Since the cellular mechanisms of sensing and responding to the mechanical stimulations at focal adhesions have not been clarified yet, we developed a micropillar substrate embedding micron-sized magnetic particles and enabling the micropillars to be cyclically deflected by a time-varied magnetic field. Using the magnetic micropillars, here we apply cyclic strain of some frequencies to a single osteoblast cell through focal adhesions and track cell migration with time-lapse observation to understand how the cell senses and responds to cyclic strain. Our data indicate that the cell may change the direction of migration to move away from the micropillar cyclically deflected in the frequency range from 0.1 to 50 Hz.

Copyright © 2015 by ASME
Topics: Osteoblasts

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