0

Full Content is available to subscribers

Subscribe/Learn More  >

A Novel Melt Electrospinning System for Studying Cell Substrate Interactions

[+] Author Affiliations
Filippos Tourlomousis, Azizbek Babakhanov, Houzhu Ding, Robert C. Chang

Stevens Institute of Technology, Hoboken, NJ

Paper No. MSEC2015-9443, pp. V002T03A006; 11 pages
doi:10.1115/MSEC2015-9443
From:
  • ASME 2015 International Manufacturing Science and Engineering Conference
  • Volume 2: Materials; Biomanufacturing; Properties, Applications and Systems; Sustainable Manufacturing
  • Charlotte, North Carolina, USA, June 8–12, 2015
  • Conference Sponsors: Manufacturing Engineering Division
  • ISBN: 978-0-7918-5683-3
  • Copyright © 2015 by ASME

abstract

Controlling cell behavior has generated immense attention in the fields of tissue engineering and regenerative medicine. Particular emphasis has been given to the creation of 3D biomimetic cellular microenvironments that replicate the complex nature of the extracellular matrix (ECM). A key factor that has not been rigorously deconstructed using scalable, layered manufacturing approaches is the structural dimension or scale aspect of in vitro culture models. Melt electrospinning represents a bio-additive manufacturing process that has been relatively under-reported. Although complex in nature, the melt electrospinning process can furnish a 3D cell delivery format with physiologically relevant 3D structural cues. In the present work, poly-ε-caprolactone (PCL) has been chosen as the biomaterial substrate. Rheological studies that guide the design phase of the reported system have been performed for the entire PCL melt processing range, implicating the governing effect of the experimental melt temperature on the scale and the topography in the final processed material. Notable challenges that arise from the nature of the process with respect to the electrospun fiber stability and resolution have been overcome through the design of a novel heating element configuration. In this paper, a reliable biofabrication process with tunable processing of the fiber diameter and alignment is reported. Fundamental parametric studies utilizing the major processing parameters demonstrate the potential for the system to precisely fabricate 3D PCL scaffolds with microstructural features.

Copyright © 2015 by ASME
Topics: Electrospinning

Figures

Tables

Interactive Graphics

Video

Country-Specific Mortality and Growth Failure in Infancy and Yound Children and Association With Material Stature

Use interactive graphics and maps to view and sort country-specific infant and early dhildhood mortality and growth failure data and their association with maternal

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging and repositioning the boxes below.

Topic Collections

Sorry! You do not have access to this content. For assistance or to subscribe, please contact us:

  • TELEPHONE: 1-800-843-2763 (Toll-free in the USA)
  • EMAIL: asmedigitalcollection@asme.org
Sign In