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Effects of Mechanical Vibration on Multilayering of Cultured Osteoblasts

[+] Author Affiliations
Toshihiko Shiraishi, Akinori Ishii, Shin Morishita

Yokohama National University, Yokohama, Japan

Paper No. IMECE2014-37731, pp. V003T03A082; 2 pages
  • ASME 2014 International Mechanical Engineering Congress and Exposition
  • Volume 3: Biomedical and Biotechnology Engineering
  • Montreal, Quebec, Canada, November 14–20, 2014
  • Conference Sponsors: ASME
  • ISBN: 978-0-7918-4646-9
  • Copyright © 2014 by ASME


This paper describes the mechanism of cell proliferation promotion by mechanical vibration focusing on multilayering of cultured osteoblasts. After osteoblasts were cultured under the mechanical vibration of 0.5 G and 12.5 Hz, the saturated cell density reached approximately twice as high as control. In the vibration group, multilayer formation of osteoblasts was observed by fluorescent microscopy in contrast with almost single layer in the control group. Fluorescent staining demonstrated that the expression of N-cadherin, which plays an important role of cell-cell adhesion, was lower under mechanical vibration than control. Therefore, the application of mechanical vibration to osteoblasts can downregulate the expression of N-cadherin, resulting in weakening of cell-cell adhesion and multilayer formation followed by promotion of cell proliferation.

Copyright © 2014 by ASME



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