0

Full Content is available to subscribers

Subscribe/Learn More  >

Understanding Temperature Profiles Experienced by Biological Samples During a Hybrid Vitrification Technique

[+] Author Affiliations
Rongheng Li, Nilay Chakraborty, Ben Q. Li

University of Michigan - Dearborn, Dearborn, MI

Paper No. IMECE2013-63947, pp. V015T16A005; 3 pages
doi:10.1115/IMECE2013-63947
From:
  • ASME 2013 International Mechanical Engineering Congress and Exposition
  • Volume 15: Safety, Reliability and Risk; Virtual Podium (Posters)
  • San Diego, California, USA, November 15–21, 2013
  • Conference Sponsors: ASME
  • ISBN: 978-0-7918-5644-4
  • Copyright © 2013 by ASME

abstract

From thermodynamic point of view, vitrification is considered as a superior preservation technique in comparison with the traditional slow-cooling cryopreservation techniques, due to formation of the glassy state in both intra and extracellular environment. While vitrification of biological samples are difficult to achieve, recently a hybrid technique involving partial desiccation of the cell samples prior to cryogenic exposure has been successfully employed to achieve vitrification. In this technique cells in monolayer attached to a substrate was suspended in a trehalose solution and then rapidly and uniformly desiccated to a low moisture content (<0.12 g of water per g of dry weight) using a spin-drying technique. The spin-dried samples were stored in liquid nitrogen (LN2) at a vitrified state (Fig. 1). It was shown that following re-warming to room temperature and re-hydration with a fully complemented cell culture medium, 51% of the spin-dried and vitrified cells survived and demonstrated normal growth characteristics. The current study further investigates the temperature profiles experienced by the cell samples during partial desiccation and cryogenic exposure to identify possible ways to improve this novel vitrification strategy. Physical Vapor Deposition technique was employed to develop glass substrate having thermocouples (2×2×2 μm) at four radial positions across the substrate to record the thermal history of the cell samples during the entire process. Efforts are undertaken to understand the uncertainties related temperature measurement spatially and with respect to time. These temperature characterization studies are important to optimize the newly developed hybrid vitrification technique for vitrification of cellular samples.

Copyright © 2013 by ASME

Figures

Tables

Interactive Graphics

Video

Country-Specific Mortality and Growth Failure in Infancy and Yound Children and Association With Material Stature

Use interactive graphics and maps to view and sort country-specific infant and early dhildhood mortality and growth failure data and their association with maternal

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging and repositioning the boxes below.

Related eBook Content
Topic Collections

Sorry! You do not have access to this content. For assistance or to subscribe, please contact us:

  • TELEPHONE: 1-800-843-2763 (Toll-free in the USA)
  • EMAIL: asmedigitalcollection@asme.org
Sign In