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Rapid Capture of Rare Cancer Cells Using a High-Performance Microfluidic Chip

[+] Author Affiliations
Weian Sheng, Tao Chen, Weihong Tan, Hugh Fan

University of Florida, Gainesville, FL

Paper No. IMECE2013-62952, pp. V010T11A003; 6 pages
doi:10.1115/IMECE2013-62952
From:
  • ASME 2013 International Mechanical Engineering Congress and Exposition
  • Volume 10: Micro- and Nano-Systems Engineering and Packaging
  • San Diego, California, USA, November 15–21, 2013
  • Conference Sponsors: ASME
  • ISBN: 978-0-7918-5639-0
  • Copyright © 2013 by ASME

abstract

Rare circulating tumor cells (CTCs) are cancer cells that detached from either a primary tumor or metastatic sites. CTCs circulate into bloodstream and become the origin of metastasis, the spread of cancer to distant organs, which is the primary cause of cancer-induced death. This paper describes our development of a microfluidic chip with unique micropillar geometry for rapid capture of CTCs from whole blood. The microscope-slide-sized microchip contains tens of thousands of isotropically-etched elliptical micropillars, which enhanced the interactions between cells and chip surfaces. The microchip was coated with DNA aptamer, an antibody-like molecule which can specifically bind with their target cells. With optimized channel geometry and flow rate, the microchip yielded a capture efficiency of >95% and a purity of >81% when capturing leukemia cells from a cell mixture. Then, the device was applied to capture colorectal tumor cells from whole blood; as few as 10 tumor cells can be efficiently isolated from 1 mL blood in 28 min. We envision that this high-performance microchip is promising for the detection, enrichment and isolation of rare CTCs, and will open up new opportunities for cancer diagnosis and monitoring cancer treatment.

Copyright © 2013 by ASME
Topics: Microfluidics , Cancer

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