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A Method for Applying High Cyclic Strain to Focal Adhesions and Measuring the Cell Response

[+] Author Affiliations
Toshihiko Shiraishi, Takuya Ohara, Shin Morishita

Yokohama National University, Yokohama, Japan

Paper No. IMECE2013-64681, pp. V03BT03A051; 2 pages
doi:10.1115/IMECE2013-64681
From:
  • ASME 2013 International Mechanical Engineering Congress and Exposition
  • Volume 3B: Biomedical and Biotechnology Engineering
  • San Diego, California, USA, November 15–21, 2013
  • Conference Sponsors: ASME
  • ISBN: 978-0-7918-5622-2
  • Copyright © 2013 by ASME

abstract

This paper describes a method by which broadband cyclic strain can be applied to focal adhesions of a cell. In recent years, evidence has been growing that focal adhesions act as mechanosensors of cells which convert mechanical force into biomechanical signaling. However, there are no effective methods by which mechanical stimulation with high frequency can be directly applied to each focal adhesion. Here we develop a micropillar substrate embedding micron-sized magnetic particles and enabling the micropillars to be deflected by external magnetic field. The combination of long and short micropillars produces the difference of deflection between them and enables the micropillars to apply strain to a cell. We verified that the micropillars responded to external magnetic field up to at least 25 Hz without phase difference. Using the magnetic micropillar substrate, we observed the cytoskeletal deformation of an osteoblast cell. The findings indicate that the present micro device can be used for investigating mechanosensing systems of a cell.

Copyright © 2013 by ASME

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