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The Protective Role of Bacillus Anthracis Exosporium in Macrophage-Mediated Killing by Nitric Oxide

[+] Author Affiliations
John Weaver, Guan-Liang Cao, Pei Tsai, Gerald Rosen

University of Maryland School of Pharmacy, Baltimore, MDUniversity of Maryland Biotechnology Institute, Baltimore, MD

Tae Jin Kang, Alan Cross

University of Maryland School of Medicine, Baltimore, MD

Kimberly Raines

University of Maryland School of Pharmacy, Baltimore, MD

Stephen Hibbs

University of Maryland Biotechnology Institute, Baltimore, MD

Les Baillie

University of Maryland Biotechnology Institute, Baltimore, MDCardiff University, Cardiff, Wales, UK

Paper No. SBC2007-176138, pp. 595-596; 2 pages
doi:10.1115/SBC2007-176138
From:
  • ASME 2007 Summer Bioengineering Conference
  • ASME 2007 Summer Bioengineering Conference
  • Keystone, Colorado, USA, June 20–24, 2007
  • Conference Sponsors: Bioengineering Division
  • ISBN: 0-7918-4798-5

abstract

The ability of the endospore-forming, gram-positive bacterium Bacillus anthracis to survive exposure to antibacterial killing mechanisms by activated macrophages is key to its germination and survival. These antibacterial killing mechanisms include, but are not limited to the generation of free radicals such as nitric oxide (•NO) and superoxide (O2) from the upregulation of inducible nitric oxide synthase (NOS 2) along with products derived from them, e.g., peroxynitrite (ONOO), as part of microbicidal activity. However questions still remain as to how these species are involved in microbial killing, specifically with respect to B. anthracis. In a previous study, we demonstrated that exposure of primary murine macrophages to sonicated B. anthracis endospores up-regulated NOS 2 and demonstrated a •NO-dependent bactericidal response, but unanswered in that study was which of the NOS 2-derived reactive oxygen species was responsible for the observed bactericidal response. Since NOS 2 also generates O2, experiments were designed to determine whether NOS 2 formed ONOO from the reaction of •NO with O2 and if so, was ONOO microbicidal toward B. anthracis.

Topics: Oxygen , Bacteria

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