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Quantitative Imaging of Nanoparticles and Intracellular Vesicle Trafficking Using Total Internal Reflection Fluorescent Microscopy (TIRFM)

[+] Author Affiliations
Charles H. Margraves, Chang K. Choi, Kenneth D. Kihm, Anthony English, Seong H. Lee, Maria Cekanova, Seung J. Baek

The University of Tennessee, Knoxville, TN

Paper No. SBC2007-176682, pp. 583-584; 2 pages
  • ASME 2007 Summer Bioengineering Conference
  • ASME 2007 Summer Bioengineering Conference
  • Keystone, Colorado, USA, June 20–24, 2007
  • Conference Sponsors: Bioengineering Division
  • ISBN: 0-7918-4798-5
  • Copyright © 2007 by ASME


Total internal reflection fluorescent microscopy (TIRFM) is a relatively well known tool used to examine the near wall region (approximately 1 μm). For years, cellular biologists have used TIRFM in a variety of experiments to examine multiple cell lines. However, much of the research has been somewhat static in nature, considering only initial and final states. With the increased ability to stain specific organelles within cells through the use of green fluorescent protein (GFP), dynamic imaging is becoming a viable solution to previously difficult problems.

Copyright © 2007 by ASME



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