0

Full Content is available to subscribers

Subscribe/Learn More  >

A 3d Histomorphometric Method for Analyses of Skeletal Tissue Mechanobiology

[+] Author Affiliations
Ryan E. Gleason

Boston University, Boston, MA

Kristy T. S. Palomares, Elise F. Morgan

Boston University, Boston, MABoston University Medical School, Boston, MA

Thomas A. Einhorn

Boston University School of Medicine, Boston, MA

Louis C. Gerstenfeld

Boston University Medical Center, Boston, MA

Paper No. SBC2007-176547, pp. 575-576; 2 pages
doi:10.1115/SBC2007-176547
From:
  • ASME 2007 Summer Bioengineering Conference
  • ASME 2007 Summer Bioengineering Conference
  • Keystone, Colorado, USA, June 20–24, 2007
  • Conference Sponsors: Bioengineering Division
  • ISBN: 0-7918-4798-5
  • Copyright © 2007 by ASME

abstract

Skeletal repair and regeneration involve a dynamic interplay of biological processes that result in spatially and temporally varying patterns of tissue formation and remodeling. For example, during bone fracture healing the cartilaginous callus that is formed initially in the fracture site is subsequently mineralized and remodeled to restore the original form and function to the injured bone. During much of this healing process, the fracture callus is comprised of a heterogeneous mixture of cartilage, fibrocartilage, multipotent mesenchymal tissue, and bone. Adding to this complexity, mechanical stimuli are known to influence the rate and type of tissues formed during skeletal healing [1]. Given the growing body of evidence that controlled mechanical stimulation may be used to enhance healing, it is of substantial interest to elucidate relationships between the distributions of local stresses and strains that develop within the healing region and the distribution of tissue types that form. While histomorphometry is a well established approach for characterizing the latter, it has historically been limited to analyses of a small number of two-dimensional sections of tissue. Such 2D sampling may be inadequate for quantitative characterization of the irregular geometry and heterogeneous composition of healing tissues. In this study, we report on a 3D histomorphometric method and apply this method to an in vivo model of skeletal repair [2] in which a bending stimulus delivered to a healing bone defect results in the formation of predominantly cartilage tissue, rather than bone.

Copyright © 2007 by ASME

Figures

Tables

Interactive Graphics

Video

Country-Specific Mortality and Growth Failure in Infancy and Yound Children and Association With Material Stature

Use interactive graphics and maps to view and sort country-specific infant and early dhildhood mortality and growth failure data and their association with maternal

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging and repositioning the boxes below.

Related eBook Content
Topic Collections

Sorry! You do not have access to this content. For assistance or to subscribe, please contact us:

  • TELEPHONE: 1-800-843-2763 (Toll-free in the USA)
  • EMAIL: asmedigitalcollection@asme.org
Sign In