Full Content is available to subscribers

Subscribe/Learn More  >

Desorption Electrospray Ionization Using a Porous Alumina Surface

[+] Author Affiliations
Ashis K. Sen, Jeff Darabi

University of South Carolina, Columbia, SC

Ranu Nayak, Daniel R. Knapp

Medical University of South Carolina, Charleston, SC

Paper No. SBC2007-176016, pp. 45-46; 2 pages
  • ASME 2007 Summer Bioengineering Conference
  • ASME 2007 Summer Bioengineering Conference
  • Keystone, Colorado, USA, June 20–24, 2007
  • Conference Sponsors: Bioengineering Division
  • ISBN: 0-7918-4798-5
  • Copyright © 2007 by ASME


Desorption electrospray ionization (DESI) is a technique used for direct sampling of a sample or an analyte deposited on a surface under ambient conditions [1]. In DESI, ionized droplets of a spray are directed towards the sample causing desorption of ions due to exchange of charge and momentum. The resulting ions are carried into an ion trap mass spectrometer and analyzed. DESI was originally demonstrated by Takats et al. [1]. They described the new method and applied the same to analyze various compounds present on a variety of surfaces. Followed by this, several researchers [1–5] have investigated on DESI for a wide range of applications including analysis of pharmaceuticals, explosives detection, natural products discovery and in vivo clinical analysis. Recently, Kauppila et al [5] have introduced porous silicon (pSi) and ultra-thin layer chromatography (UTLC) plates for DESI-MS. Similar or improved sensitivities were obtained with pSi and UTLC surfaces as compared to PMMA and PTFE surfaces. This work presents use of a nanoporous alumina surface [6] for DESI – MS. The DESI – MS performance of nanoporous alumina surface is compared with that of PMMA, which is a popular surface in previous DESI-MS experiments. Optimized operating conditions were determined for the surfaces using BSA tryptic digest as the sample. The results show that the nanoporous alumina surface offers significantly higher ion intensity as compared to the other surfaces.

Copyright © 2007 by ASME



Interactive Graphics


Country-Specific Mortality and Growth Failure in Infancy and Yound Children and Association With Material Stature

Use interactive graphics and maps to view and sort country-specific infant and early dhildhood mortality and growth failure data and their association with maternal

Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging and repositioning the boxes below.

Related eBook Content
Topic Collections

Sorry! You do not have access to this content. For assistance or to subscribe, please contact us:

  • TELEPHONE: 1-800-843-2763 (Toll-free in the USA)
  • EMAIL: asmedigitalcollection@asme.org
Sign In