0

Full Content is available to subscribers

Subscribe/Learn More  >

Dynamic Hydrostatic Pressurization Differentially Regulates Extracellular Matrix Elaboration by Bovine Inner and Outer Annulus Fibrosus Cells Seeded on 3-D Polymer Scaffolds

[+] Author Affiliations
Anna T. Reza, Steven B. Nicoll

Bioengineering, University of Pennsylvania, Philadelphia, PA

Paper No. SBC2007-176539, pp. 1029-1030; 2 pages
doi:10.1115/SBC2007-176539
From:
  • ASME 2007 Summer Bioengineering Conference
  • ASME 2007 Summer Bioengineering Conference
  • Keystone, Colorado, USA, June 20–24, 2007
  • Conference Sponsors: Bioengineering Division
  • ISBN: 0-7918-4798-5
  • Copyright © 2007 by ASME

abstract

Current surgical treatments for intervertebral disc (IVD) degeneration result in decreased mobility of the spine [1]. A tissue engineering approach may provide an alternative that restores both IVD structure and function. The IVD is comprised of three distinct regions: the outer annulus fibrosus (OA), inner annulus fibrosus (IA), and the nucleus pulposus (NP). Each of the cell populations within these regions possess unique phenotypic properties that are greatly influenced by environmental factors, such as the surrounding 3-D extracellular matrix (ECM) and mechanical loading (i.e., hydrostatic pressurization) [2]. As such, both the 3-D scaffold and in vitro culture conditions may have marked effects on the development of tissue-engineered IVD constructs. Although the influence of mechanical loading on IVD cells and explants has been investigated, no prior studies have examined the impact of hydrostatic pressurization on OA and IA cells in 3-D culture. Therefore, the objective of this study was to determine the effects of dynamic hydrostatic pressurization on OA and IA cells seeded on 3-D fibrous poly(glycolic acid)-poly(L-lactic acid) (PGA-PLLA) scaffolds. We hypothesized that the application of hydrostatic pressure would promote increased production of type II collagen and chondroitin sulfate proteoglycan in both OA- and IA-seeded constructs.

Copyright © 2007 by ASME

Figures

Tables

Interactive Graphics

Video

Country-Specific Mortality and Growth Failure in Infancy and Yound Children and Association With Material Stature

Use interactive graphics and maps to view and sort country-specific infant and early dhildhood mortality and growth failure data and their association with maternal

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging and repositioning the boxes below.

Related eBook Content
Topic Collections

Sorry! You do not have access to this content. For assistance or to subscribe, please contact us:

  • TELEPHONE: 1-800-843-2763 (Toll-free in the USA)
  • EMAIL: asmedigitalcollection@asme.org
Sign In