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Real-Time Image Processing for Locating Veins in Mouse Tails

[+] Author Affiliations
Yen-Chi Chang, Brittany N. Berry-Pusey, Tsu-Chin Tsao, Arion F. Chatziioannou

University of California, Los Angeles, CA

Paper No. DSCC2013-4023, pp. V002T29A004; 7 pages
  • ASME 2013 Dynamic Systems and Control Conference
  • Volume 2: Control, Monitoring, and Energy Harvesting of Vibratory Systems; Cooperative and Networked Control; Delay Systems; Dynamical Modeling and Diagnostics in Biomedical Systems; Estimation and Id of Energy Systems; Fault Detection; Flow and Thermal Systems; Haptics and Hand Motion; Human Assistive Systems and Wearable Robots; Instrumentation and Characterization in Bio-Systems; Intelligent Transportation Systems; Linear Systems and Robust Control; Marine Vehicles; Nonholonomic Systems
  • Palo Alto, California, USA, October 21–23, 2013
  • Conference Sponsors: Dynamic Systems and Control Division
  • ISBN: 978-0-7918-5613-0
  • Copyright © 2013 by ASME


This paper develops an efficient vision-based real-time vein detection algorithm for preclinical vascular insertions. Mouse tail vein injections perform a routine but critical step in most preclinical applications. Compensating for poor manual injection stability and high skill requirements, Vascular Access System (VAS) has been developed so a trained technician can manually command the system to perform needle insertions and monitor the operation through a near-infrared camera. However, VAS’ vein detection algorithm requires much computation and is, therefore, difficult to reflect the real-time tail movement during an insertion. Furthermore, the detection performance is often disturbed by tail hair and skin pigmentation. In this work, an effective noise filtering algorithm is proposed based on convex optimization. Effectively eliminating false-positive detections and preserving cross-sectional continuity, this algorithm provides vein detection results approximately every 200 ms at the presence of tail hair and skin pigmentation. This developed real-time tail vein detection method is able to capture the tail movement during insertion, therefore allow for the development of an automated Vascular Access System (A-VAS) for preclinical injections.

Copyright © 2013 by ASME



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