0

Full Content is available to subscribers

Subscribe/Learn More  >

Quantifying the Growth Rate and Morphology of Ice Crystals Growing in Cryoprotectants via High-Speed Camera and Cryo-Microscope

[+] Author Affiliations
Xu Xue, Zhi Zhu He

Chinese Academy of Sciences, Beijing, China

Jing Liu

Chinese Academy of Sciences, Beijing, ChinaTsinghua University, Beijing, China

Paper No. MNHMT2013-22134, pp. V001T08A002; 4 pages
doi:10.1115/MNHMT2013-22134
From:
  • ASME 2013 4th International Conference on Micro/Nanoscale Heat and Mass Transfer
  • ASME 2013 4th International Conference on Micro/Nanoscale Heat and Mass Transfer
  • Hong Kong, China, December 11–14, 2013
  • Conference Sponsors: Heat Transfer Division
  • ISBN: 978-0-7918-3615-4
  • Copyright © 2013 by ASME

abstract

Recently, several significant progresses have been made on the studies of extracellular and intracellular ice formation based on high-speed camera and cryomicroscope. This experimental methodology could accurately capture rapid formation process of ice crystals at micro-scale. In this paper, we are dedicated to quantify and comparatively investigate the growth rate and morphology of ice crystals growing in DMSO, sucrose and trehalose, respectively via high-speed camera and cryo-microscope. Several impact factors such as the concentration of cryoprotectants and the cooling rate have been investigated. The results indicate that the species and concentration of cryoprotectants and the cooling rate could significantly affect the growth rate and morphology of ice crystals. DMSO is better than trehalose and sucrose as cryoprotectant because of its molecular structure. This work may enhance current understanding of the factors for ice crystals formation and help optimize the cryopreservation process in the near future.

Copyright © 2013 by ASME
Topics: Crystals , Ice , Microscopes

Figures

Tables

Interactive Graphics

Video

Country-Specific Mortality and Growth Failure in Infancy and Yound Children and Association With Material Stature

Use interactive graphics and maps to view and sort country-specific infant and early dhildhood mortality and growth failure data and their association with maternal

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging and repositioning the boxes below.

Related eBook Content
Topic Collections

Sorry! You do not have access to this content. For assistance or to subscribe, please contact us:

  • TELEPHONE: 1-800-843-2763 (Toll-free in the USA)
  • EMAIL: asmedigitalcollection@asme.org
Sign In