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Inertial Microfluidics for Separation and Detection of Tumor Cells

[+] Author Affiliations
Jiashu Sun

National Center for NanoScience and Technology, Beijing, China

Paper No. MNHMT2013-22036, pp. V001T01A001; 5 pages
doi:10.1115/MNHMT2013-22036
From:
  • ASME 2013 4th International Conference on Micro/Nanoscale Heat and Mass Transfer
  • ASME 2013 4th International Conference on Micro/Nanoscale Heat and Mass Transfer
  • Hong Kong, China, December 11–14, 2013
  • Conference Sponsors: Heat Transfer Division
  • ISBN: 978-0-7918-3615-4
  • Copyright © 2013 by ASME

abstract

We report on the development of a curved microfluidic channel that allows rapid and continuous size-based rare tumor cell separation from blood in a label-free manner by exploiting the hydrodynamic effects. The separated tumor cells are trapped and enriched on an integrated polycarbonate filter glued on top of the outlet reservoir of microchannels. CK19 mRNA of MCF-7 cells are detected by loop-mediated isothermal amplification (LAMP).

Copyright © 2013 by ASME

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