0

Full Content is available to subscribers

Subscribe/Learn More  >

Engineered Fibrillar Microenvironments With Controllable Architecture and Mechanics for Studying Cellular Stiffness Sensing

[+] Author Affiliations
Brendon M. Baker, Britta Trappmann, Iris L. Kim, Jason A. Burdick, Christopher S. Chen

University of Pennsylvania, Philadelphia, PA

Paper No. SBC2013-14804, pp. V01AT22A006; 2 pages
doi:10.1115/SBC2013-14804
From:
  • ASME 2013 Summer Bioengineering Conference
  • Volume 1A: Abdominal Aortic Aneurysms; Active and Reactive Soft Matter; Atherosclerosis; BioFluid Mechanics; Education; Biotransport Phenomena; Bone, Joint and Spine Mechanics; Brain Injury; Cardiac Mechanics; Cardiovascular Devices, Fluids and Imaging; Cartilage and Disc Mechanics; Cell and Tissue Engineering; Cerebral Aneurysms; Computational Biofluid Dynamics; Device Design, Human Dynamics, and Rehabilitation; Drug Delivery and Disease Treatment; Engineered Cellular Environments
  • Sunriver, Oregon, USA, June 26–29, 2013
  • Conference Sponsors: Bioengineering Division
  • ISBN: 978-0-7918-5560-7
  • Copyright © 2013 by ASME

abstract

The mechanical properties of the extracellular matrix (ECM) have emerged as fundamental players in numerous basic cellular functions such as spreading, migration, proliferation and differentiation, thus impacting many biological processes including embryonic development, adult tissue homeostasis, and disease pathogenesis such as fibrosis and cancer [1,2,3]. Synthetic matrices have been crucial to studying the effect of mechanics on cell behavior, as they allow for precise control of mechanical properties over a wide stiffness range, unachievable in vivo or in many naturally derived material systems. Seminal work employing polyacrylamide hydrogels of varying stiffness to direct the differentiation of human mesenchymal stem cells concluded that the bulk modulus, a measure of the material’s resistance to uniform deformation, is a defining parameter influencing cell function [4]. While much current effort aims to shed light on the molecular mechanisms governing stiffness sensing, existing knowledge is limited by the dissimilarity between the simple hydrogel surfaces employed in these studies and the topographically and mechanically more complex ECM cells routinely reside within in vivo. In contrast to the flat expanse of cell adhesive ligand and linear elastic, continuum behavior of typical gel systems, within the body, cell-scale mechanics and ligand availability are entwined, as both are defined by the presence and organization of the proteins that compose the surrounding ECM. The structure of native ECMs vary but largely are fibrillar, given that collagen comprises approximately 25% of the human body by mass. Thus, there remains a significant need for engineered fibrillar materials that afford precise and independent control of architectural features and resulting mechanical properties for application in cell biology. In this work, we establish a novel material system towards this end.

Copyright © 2013 by ASME
Topics: Stiffness

Figures

Tables

Interactive Graphics

Video

Country-Specific Mortality and Growth Failure in Infancy and Yound Children and Association With Material Stature

Use interactive graphics and maps to view and sort country-specific infant and early dhildhood mortality and growth failure data and their association with maternal

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging and repositioning the boxes below.

Related eBook Content
Topic Collections

Sorry! You do not have access to this content. For assistance or to subscribe, please contact us:

  • TELEPHONE: 1-800-843-2763 (Toll-free in the USA)
  • EMAIL: asmedigitalcollection@asme.org
Sign In