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High Performance Microfluidic-Based DNA Isolation Chip

[+] Author Affiliations
Jeff Darabi

Southern Illinois University, Edwardsville, IL

Paper No. SBC2013-14522, pp. V01AT20A022; 2 pages
doi:10.1115/SBC2013-14522
From:
  • ASME 2013 Summer Bioengineering Conference
  • Volume 1A: Abdominal Aortic Aneurysms; Active and Reactive Soft Matter; Atherosclerosis; BioFluid Mechanics; Education; Biotransport Phenomena; Bone, Joint and Spine Mechanics; Brain Injury; Cardiac Mechanics; Cardiovascular Devices, Fluids and Imaging; Cartilage and Disc Mechanics; Cell and Tissue Engineering; Cerebral Aneurysms; Computational Biofluid Dynamics; Device Design, Human Dynamics, and Rehabilitation; Drug Delivery and Disease Treatment; Engineered Cellular Environments
  • Sunriver, Oregon, USA, June 26–29, 2013
  • Conference Sponsors: Bioengineering Division
  • ISBN: 978-0-7918-5560-7
  • Copyright © 2013 by ASME

abstract

Magnetic separation is one of the effective ways to separate specific biological entities such as DNA/RNA, bacteria, and cells from their native environment for subsequent downstream analysis. The process involves the labeling of the desired biological entity with magnetic beads followed by separating the tagged entities via a magnetic separation device. In conventional tube-based magnetic separation, magnetically labeled biological entities are retained on the inner wall of the tube by applying an external magnet, while the supernatant is decanted off. Removing the tube from the magnetic field enables resuspension of the target entity. Although widely used, there are limitations to the conventional magnetic separation method. For example, there is a significant sample loss due to multiple sample handling, washing, and transfer. In addition, manual magnetic separation systems are labor intensive and their effectiveness is user-dependent.

Copyright © 2013 by ASME
Topics: Microfluidics , DNA

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