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Numerical Investigation of a Novel Microfluidic System and its Application in Achieving Ultra-Fast Cooling/Warming Rates for Cell Vitrification Cryopreservation

[+] Author Affiliations
Xiaoming Zhou, Pingan Du

University of Electronic Science and Technology of China, Chengdu, Sichuan, China

Xin Liang, Zhiquan Shu, Dayong Gao

University of Washington, Seattle, WA

Paper No. SBC2013-14077, pp. V01AT20A005; 2 pages
  • ASME 2013 Summer Bioengineering Conference
  • Volume 1A: Abdominal Aortic Aneurysms; Active and Reactive Soft Matter; Atherosclerosis; BioFluid Mechanics; Education; Biotransport Phenomena; Bone, Joint and Spine Mechanics; Brain Injury; Cardiac Mechanics; Cardiovascular Devices, Fluids and Imaging; Cartilage and Disc Mechanics; Cell and Tissue Engineering; Cerebral Aneurysms; Computational Biofluid Dynamics; Device Design, Human Dynamics, and Rehabilitation; Drug Delivery and Disease Treatment; Engineered Cellular Environments
  • Sunriver, Oregon, USA, June 26–29, 2013
  • Conference Sponsors: Bioengineering Division
  • ISBN: 978-0-7918-5560-7
  • Copyright © 2013 by ASME


Background: During conventional cryopreservation, cells are often damaged by ice formation. An alternative approach is to use ultra-rapid cooling rates to achieve vitrification of cell suspension. A variety of systems and methods have been developed, e.g. open pulled straws (OPS), closed pulled straws (CPS), cryo-loop and micro-droplets, by which cooling/warming rates up to 104∼105 °C/min can be achieved[1]. Although these methods have been well used in some areas, they are still far from perfect because (1) the cooling/warming rates are still not fast enough and thus high-concentration cryoprotectants should be used; (2) the sample size is often seriously limited.

Copyright © 2013 by ASME



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