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Cellular Traction Forces Measured With Microposts Made by Hot Embossing of Polystyrene

[+] Author Affiliations
Kevin S. Bielawski, Nathan J. Sniadecki

University of Washington, Seattle, WA

Paper No. SBC2013-14568, pp. V01AT17A021; 2 pages
doi:10.1115/SBC2013-14568
From:
  • ASME 2013 Summer Bioengineering Conference
  • Volume 1A: Abdominal Aortic Aneurysms; Active and Reactive Soft Matter; Atherosclerosis; BioFluid Mechanics; Education; Biotransport Phenomena; Bone, Joint and Spine Mechanics; Brain Injury; Cardiac Mechanics; Cardiovascular Devices, Fluids and Imaging; Cartilage and Disc Mechanics; Cell and Tissue Engineering; Cerebral Aneurysms; Computational Biofluid Dynamics; Device Design, Human Dynamics, and Rehabilitation; Drug Delivery and Disease Treatment; Engineered Cellular Environments
  • Sunriver, Oregon, USA, June 26–29, 2013
  • Conference Sponsors: Bioengineering Division
  • ISBN: 978-0-7918-5560-7
  • Copyright © 2013 by ASME

abstract

Polydimethylsiloxane (PDMS) has become a highly utilized tool to study the forces that cells generate, although, outside of lab on chip devices, it is not widely used and requires protein coatings to encourage cell adhesion1. Furthermore, PDMS suffers from changes in composition and stiffness with different curing conditions2. Alternatively, polystyrene is a common substrate that promotes cell adhesion and has mostly consistent properties; however, polystyrene is typically challenging to form without special equipment and expensive molds. Previously, a hot embossing method3 has been proposed to manufacture polystyrene devices using a PDMS negative mold and polystyrene chips. A moderate amount of pressure and temperatures above the glass transition temperature of polystyrene enable the polystyrene to flow into the mold. In this paper, we fabricate microposts out of polystyrene and successfully seed cells on top of the posts.

Copyright © 2013 by ASME
Topics: Embossing , Traction

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