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Steady Flow Preconditioning of Endothelial Outgrowth Cells on Ex Vivo and In Vivo ePTFE Grafts

[+] Author Affiliations
D. E. J. Anderson, J. J. Glynn, M. T. Hinds

Oregon Health and Science University, Portland, OR

Paper No. SBC2013-14206, pp. V01AT15A003; 2 pages
doi:10.1115/SBC2013-14206
From:
  • ASME 2013 Summer Bioengineering Conference
  • Volume 1A: Abdominal Aortic Aneurysms; Active and Reactive Soft Matter; Atherosclerosis; BioFluid Mechanics; Education; Biotransport Phenomena; Bone, Joint and Spine Mechanics; Brain Injury; Cardiac Mechanics; Cardiovascular Devices, Fluids and Imaging; Cartilage and Disc Mechanics; Cell and Tissue Engineering; Cerebral Aneurysms; Computational Biofluid Dynamics; Device Design, Human Dynamics, and Rehabilitation; Drug Delivery and Disease Treatment; Engineered Cellular Environments
  • Sunriver, Oregon, USA, June 26–29, 2013
  • Conference Sponsors: Bioengineering Division
  • ISBN: 978-0-7918-5560-7
  • Copyright © 2013 by ASME

abstract

Endothelialization of vascular graft materials is a promising approach for improving the in vivo performance of vascular grafts, particularly for small diameter applications of less than 4 mm. The ability to incorporate a native endothelium onto a graft may reduce the thrombosis and intimal hyperplasia that limits long-term clinical success of these small diameter grafts. Endothelial outgrowth cells (EOCs), which are isolated from whole blood and expand rapidly in vitro, provide an autologous cell source capable of developing into a biologically active endothelial layer. A preconditioning step may enhance EOCs’ performance on vascular grafts. Mature endothelial cells, isolated from vascular walls, are known to decrease expression of pro-thrombotic and pro-inflammatory markers when exposed to steady fluid shear stress, compared to cells under disturbed flow conditions or static culture. This study examined the hypothesis that steady flow preconditioning of EOCs reduces their in vitro markers of thrombosis and inflammation, reduces platelet and fibrin accumulation on EOC–coated ePTFE grafts in an ex vivo shunt, and reduces initial hyperplasia on EOC–coated ePTFE grafts in an in vivo graft implant. This work was performed using well-established, non-human primate models for testing EOC-coated ePTFE grafts ex vivo and in vivo. These conditions represent a clinically-relevant cell source and biomaterial for determining the effects of fluid shear stress preconditioning on graft performance.

Copyright © 2013 by ASME
Topics: Flow (Dynamics)

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