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Effect of CPA Transmembrane Flux on Cell Volume Change During Freezing and its Application in Biopreservation

[+] Author Affiliations
Gang Zhao

University of Science and Technology of China, Hefei, China

Dayong Gao

University of Science and Technology of China, Hefei, ChinaUniversity of Washington, Seattle, WA

Paper No. SBC2013-14231, pp. V01AT07A011; 2 pages
doi:10.1115/SBC2013-14231
From:
  • ASME 2013 Summer Bioengineering Conference
  • Volume 1A: Abdominal Aortic Aneurysms; Active and Reactive Soft Matter; Atherosclerosis; BioFluid Mechanics; Education; Biotransport Phenomena; Bone, Joint and Spine Mechanics; Brain Injury; Cardiac Mechanics; Cardiovascular Devices, Fluids and Imaging; Cartilage and Disc Mechanics; Cell and Tissue Engineering; Cerebral Aneurysms; Computational Biofluid Dynamics; Device Design, Human Dynamics, and Rehabilitation; Drug Delivery and Disease Treatment; Engineered Cellular Environments
  • Sunriver, Oregon, USA, June 26–29, 2013
  • Conference Sponsors: Bioengineering Division
  • ISBN: 978-0-7918-5560-7
  • Copyright © 2013 by ASME

abstract

Theoretical models for prediction of cell dehydration during addition/removal of cryoprotective agents (CPAs) or during freezing sprung up like mushrooms. These models are powerful for qualitative analysis of “solution injury” or “osmotic injury”, and furthermore, optimization of the complex processes involved in cryopreservation.

Copyright © 2013 by ASME
Topics: Freezing

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