0

Full Content is available to subscribers

Subscribe/Learn More  >

Non-Invasive Sorting of Lipid Producing Microalgae With Dielectrophoresis Using Microelectrodes

[+] Author Affiliations
Alisha R. Schor, Cullen R. Buie

Massachusetts Institute of Technology, Cambridge, MA

Paper No. IMECE2012-88317, pp. 701-707; 7 pages
doi:10.1115/IMECE2012-88317
From:
  • ASME 2012 International Mechanical Engineering Congress and Exposition
  • Volume 9: Micro- and Nano-Systems Engineering and Packaging, Parts A and B
  • Houston, Texas, USA, November 9–15, 2012
  • Conference Sponsors: ASME
  • ISBN: 978-0-7918-4525-7
  • Copyright © 2012 by ASME

abstract

In order to advance the algae biofuel industry, we are constructing a dielectrophoretic, single-cell sorter that selects algae based on lipid content. This tool can lower production costs by aiding in strain selection, online culture monitoring, or directed evolution studies. Dielectrophoresis (DEP) is the polarization of particles or cells in a non-uniform electric field, which leads to a Coulomb force on the cell. Lipids and cell cytoplasm have vastly different dielectric properties. Therefore, as a cell accumulates lipid, we predict a change in the overall DEP response. Our models show that in algae culture medium, we should be able to distinguish between high and low lipid content cells at frequencies above 100 MHz. This was confirmed by experiments, in which high and low lipid cultures of Neochloris oleoabundans have DEP crossover frequencies of 190 MHz and 125 MHz, respectively. We have also fabricated a proof-of-concept device validating that cells can be manipulated under DEP. However, in order to achieve sorting, we will require higher frequencies as well as a modified design to eliminate non-uniformities in the electric field through the channel height.

Copyright © 2012 by ASME

Figures

Tables

Interactive Graphics

Video

Country-Specific Mortality and Growth Failure in Infancy and Yound Children and Association With Material Stature

Use interactive graphics and maps to view and sort country-specific infant and early dhildhood mortality and growth failure data and their association with maternal

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging and repositioning the boxes below.

Related eBook Content
Topic Collections

Sorry! You do not have access to this content. For assistance or to subscribe, please contact us:

  • TELEPHONE: 1-800-843-2763 (Toll-free in the USA)
  • EMAIL: asmedigitalcollection@asme.org
Sign In