Full Content is available to subscribers

Subscribe/Learn More  >

Analysis of Ciliary Motion and the Axonemal Structure in the Mouse Respiratory Cilia

[+] Author Affiliations
Hironori Ueno

Tohoku University, Sendai, Miyagi, JapanPaul Scherrer Institute (PSI), Villligen PSI, Switzerland

Takuji Ishikawa, Kohsuke Gonda, Takami Yamaguchi

Tohoku University, Sendai, Miyagi, Japan

Khanh Huy Bui, Takashi Ishikawa

Paul Scherrer Institute (PSI), Villligen PSI, Switzerland

Paper No. SBC2012-80232, pp. 1259-1260; 2 pages
  • ASME 2012 Summer Bioengineering Conference
  • ASME 2012 Summer Bioengineering Conference, Parts A and B
  • Fajardo, Puerto Rico, USA, June 20–23, 2012
  • Conference Sponsors: Bioengineering Division
  • ISBN: 978-0-7918-4480-9
  • Copyright © 2012 by ASME


Mucociliary clearance on the surface of the tracheal lumen is an important component of lung defense against dust mites and viruses. However, the axonemal structure that achieves effective ciliary motion and the mechanisms by which discretely distributed ciliary cells generate directional flow are unknown. In this study, we examined individual ciliary motion with 7–9-nm spatial precision by labeling the ciliary tip with quantum dots, and detected an asymmetric beating pattern. Cryo-electron tomography revealed that the densities of two inner dynein arms were missing from at least two doublet microtubules in the axonemal structure. Although the flow directions generated by individual ciliated cells were unsteady and diverse, the time- and space-averaged velocity field was found to be directional. These results indicate that the asymmetric ciliary motion is driven by the asymmetric axonemal structure, and it generates overall directional flow from the lungs to the oropharynx on sparsely distributed ciliated cells.

Copyright © 2012 by ASME



Interactive Graphics


Country-Specific Mortality and Growth Failure in Infancy and Yound Children and Association With Material Stature

Use interactive graphics and maps to view and sort country-specific infant and early dhildhood mortality and growth failure data and their association with maternal

Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging and repositioning the boxes below.

Related eBook Content
Topic Collections

Sorry! You do not have access to this content. For assistance or to subscribe, please contact us:

  • TELEPHONE: 1-800-843-2763 (Toll-free in the USA)
  • EMAIL: asmedigitalcollection@asme.org
Sign In