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Nanostructure Processed on Culture Plate Improves Cell Adhesion

[+] Author Affiliations
Lisa Suganuma, Yasuhiro Tanaka, Nobuyuki Moronuki

Tokyo Metropolitan University, Tokyo, Japan

Hiromichi Fujie

Tokyo Metropolitan University, Tokyo, JapanKogakuin University, Tokyo, Japan

Hiroki Sudama, Yoshihide Sato, Kenji Suzuki

Kogakuin University, Tokyo, Japan

Norimasa Nakamura

Osaka University Graduate School of Medicine, Osaka, Japan

Paper No. SBC2011-53753, pp. 953-954; 2 pages
  • ASME 2011 Summer Bioengineering Conference
  • ASME 2011 Summer Bioengineering Conference, Parts A and B
  • Farmington, Pennsylvania, USA, June 22–25, 2011
  • Conference Sponsors: Bioengineering Division
  • ISBN: 978-0-7918-5458-7
  • Copyright © 2011 by ASME


Ligaments and tendons have superior functions, but their healing capacities are limited. We have been developing a novel tissue-engineering technique for the repair of ligaments and tendons which involve stem cell-based self-assembled tissues (scSAT) derived from synovium[1]. For biological reconstruction of soft tissues, it is required for the scSAT to have high tensile strength. Our previous study indicted that, when the scSAT was cultured under high cell density condition, the tensile strength of the scSAT become higher than that cultured under low density condition[2]. However, the scSAT had a significant tendency to detach naturally from the culture dish with increasing cell density. Therefore, we expect that the mechanical property of the scSAT improves by enhancing the cell adhesion to culture plates. Previous studies suggested that nanostructure processed on culture dish affected cell adhesion [3, 4]. In the present study, nanostructure was processed on a silicon wafer using a nanoprocessing technology, and the structure was replicated to a polydimethylsiloxane (PDMS) plate. Human synovium-derived mesenchymal stem cells were cultured on the plate, and cell adhesion and morphological observation were performed.

Copyright © 2011 by ASME



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