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Amplification-Free DNA Detection Using a Microtip-Sensor Decorated With LNA Probes for Rapid TB Screening

[+] Author Affiliations
Shinnosuke Inoue, Woon-Hong Yeo, Jong-Hoon Kim, Jae-Hyun Chung, Dayong Gao

University of Washington, Seattle, WA

Kyong-Hoon Lee, Kieseok Oh

NanoFacture, Inc., Bellevue, WA

Gerard Cangelosi

Seattle Biomedical Research Institute, Seattle, WA

Paper No. IMECE2011-64378, pp. 937-939; 3 pages
  • ASME 2011 International Mechanical Engineering Congress and Exposition
  • Volume 2: Biomedical and Biotechnology Engineering; Nanoengineering for Medicine and Biology
  • Denver, Colorado, USA, November 11–17, 2011
  • Conference Sponsors: ASME
  • ISBN: 978-0-7918-5488-4
  • Copyright © 2011 by ASME


Tuberculosis (TB) is an epidemic affecting one-third of the world’s population, mostly in developing and low-resource settings. People having active pulmonary TB are considered highly infectious; therefore, it is critical to identify and treat these patients rapidly before spreading to others. However, the most reliable TB diagnostic methods of bacterial culture or nucleic acid amplification are time-consuming and expensive. The challenge of TB diagnosis lies in highly sensitive and specific screening with low cost. Here, we present an LNA-modified microtip-sensor, which is capable of selectively detecting low-abundance DNA from bacteria. When genomic DNA of Bacillus Calmette-Guérin (BCG, a surrogate marker of Mycobacterium bovis), and genomic DNA of Staphylococcus epidermidis (S. epi) are used, the microtip-sensor yields the detection limit of 1,000 copies/mL within 20 minutes. The high sensitivity and specificity approaching nucleic acid amplification methods can potentially overcome the current challenges for rapid TB screening.

Copyright © 2011 by ASME
Topics: Sensors , Probes , DNA



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