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Study of Cells Attachment Using Impedance Spectroscopy Technique

[+] Author Affiliations
Anis N. Nordin, Maizirwan Mel

International Islamic University Malaysia, Kuala Lumpur, Malaysia

S. M. Arifuzzaman, Ioana Voiculescu

City College of New York, New York, NY

David Spray

Albert Einstein College of Medicine, New York, NY

Paper No. IMECE2011-62919, pp. 931-935; 5 pages
doi:10.1115/IMECE2011-62919
From:
  • ASME 2011 International Mechanical Engineering Congress and Exposition
  • Volume 2: Biomedical and Biotechnology Engineering; Nanoengineering for Medicine and Biology
  • Denver, Colorado, USA, November 11–17, 2011
  • Conference Sponsors: ASME
  • ISBN: 978-0-7918-5488-4
  • Copyright © 2011 by ASME

abstract

In this paper we report on the electrical impedance spectroscopy characterization of the attachment of two mammalian cell lines: endothelial cells (RFPEC) and fibroblast cells (V79). For electrical cell–substrate impedance sensing (ECIS) of the endothelial cells a commercially available eight-well cell culture impedance array (ECIS-8W1E) was used. The impedance measurements were recorded with cell culture medium (without cells) and with endothelial cells or fibroblast cells in the culture medium over a frequency range from 100 Hz to 100 kHz. The impedance measurements were compared to the equivalent circuit model. The impedance measurements were also simulated using COMSOL Multiphysics™, a commercially available modeling package.

Copyright © 2011 by ASME

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