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Investigation of Mechanism of Proliferation Promotion of Cultured Osteoblasts by Mechanical Vibration

[+] Author Affiliations
Toshihiko Shiraishi, Kazutaka Ohashi, Shin Morishita

Yokohama National University, Yokohama, Japan

Ryohei Takeuchi

Yokohama City University School of Medicine, Yokohama, Japan

Paper No. IMECE2011-64845, pp. 691-692; 2 pages
doi:10.1115/IMECE2011-64845
From:
  • ASME 2011 International Mechanical Engineering Congress and Exposition
  • Volume 2: Biomedical and Biotechnology Engineering; Nanoengineering for Medicine and Biology
  • Denver, Colorado, USA, November 11–17, 2011
  • Conference Sponsors: ASME
  • ISBN: 978-0-7918-5488-4
  • Copyright © 2011 by ASME

abstract

This paper describes effects of mechanical vibration on osteoblasts. Their cell proliferation was investigated when sinusoidal inertia force was applied to the cells. After the cells were cultured in culture plates in a CO2 incubator for one day and adhered on the cultured plane, vibration group of the culture plates was set on an aluminum plate attached to a exciter and cultured under sinusoidal excitation of 0.5 G and 12.5 Hz for 24 hours a day in another incubator separated from non-vibration group during 21 days of culture. The time evolution of cell density was obtained by counting the number of cells with a hemocytometer. The phase contrast microscopic images and the cross-sectional confocal microscopic images of cells were captured under an inverted microscope system. As a result, it is found that the mechanical vibration elongated the pseudopods of cells to enhance their mobility resulting in promoting multilayer of the cells.

Copyright © 2011 by ASME

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