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Protein Adsorption in a Continuous Flow Microchannel Environment

[+] Author Affiliations
Pin-Chuan Chen, Hong Wang, Daniel S. Park, Sunggook Park, Dimitris E. Nikitopoulos, Steven A. Soper, Michael C. Murphy

Louisiana State University, Baton Rouge, LA

Paper No. IMECE2008-68094, pp. 249-253; 5 pages
doi:10.1115/IMECE2008-68094
From:
  • ASME 2008 International Mechanical Engineering Congress and Exposition
  • Volume 2: Biomedical and Biotechnology Engineering
  • Boston, Massachusetts, USA, October 31–November 6, 2008
  • Conference Sponsors: ASME
  • ISBN: 978-0-7918-4863-0 | eISBN: 978-0-7918-3840-2
  • Copyright © 2008 by ASME

abstract

Protein adsorption is a critical issue in microfluidic devices especially for those reactions depending on proteins like the polymerase chain reaction (PCR). Understanding protein absorption phenomena in different geometry microchannels and evaluating the efficiency of dynamic coating, which has been using as a method to prevent protein adsorption, are important tasks. Two different sets of microchannels were designed and fabricated on polymers. Bovine serum albumin (BSA) was used as a model protein for quantification of and monitoring the protein loss in different microchannel geometries. Up to 58% of the BSA was lost after flowing a 2030 mm long microchannel. The BSA adsorption rate changed along the microchannel. Smaller microchannels required a longer time to achieve protein saturation point. Dynamic coating was shown to be a time consuming and inefficient method to prevent protein adsorption in a continuous flow environment.

Copyright © 2008 by ASME

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