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Comparison of Different Metal Film Thicknesses of COC-Substrate Polymerase Chain Reaction Chips With Single-Side and Double-Side Heaters

[+] Author Affiliations
Yung-Chiang Chung, Li-Wei Lai, Wei-Jen Liao

Ming Chi University of Technology, Taipei, Taiwan, R.O.C.

Lung-Jieh Yang

Tamkang University, Taipei, Taiwan, R.O.C.

Paper No. IMECE2009-10229, pp. 435-437; 3 pages
  • ASME 2009 International Mechanical Engineering Congress and Exposition
  • Volume 2: Biomedical and Biotechnology Engineering
  • Lake Buena Vista, Florida, USA, November 13–19, 2009
  • Conference Sponsors: ASME
  • ISBN: 978-0-7918-4375-8 | eISBN: 978-0-7918-3863-1
  • Copyright © 2009 by ASME


We fabricated PCR chips of different metal film thicknesses. The PCR machine with/without top heater and the single-side/double-side heater PCR chips were compared. The substrate was transparent COC (Cyclic Olefin Copolymers), and the gold film was fabricated on the substrate by evaporation without any adhesive layer. Then the results after polymerase chain reaction for the DNA of Escherichia coli TG-1 cell were obtained. The concentrations of amplifying 225 bps DNA were 0.78–0.82 ng/μl (64% of the result of PCR machine).

Copyright © 2009 by ASME
Topics: Metallic films , Chain



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