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Diffusion-Mediated Production of Protein Gradients by Way of Variable Depth Hydrogel Microstamps

[+] Author Affiliations
Dewayne L. Thompson, Ashley E. Madon, Christine A. Trinkle

University of Kentucky, Lexington, KY

Paper No. IMECE2009-11292, pp. 255-256; 2 pages
doi:10.1115/IMECE2009-11292
From:
  • ASME 2009 International Mechanical Engineering Congress and Exposition
  • Volume 2: Biomedical and Biotechnology Engineering
  • Lake Buena Vista, Florida, USA, November 13–19, 2009
  • Conference Sponsors: ASME
  • ISBN: 978-0-7918-4375-8 | eISBN: 978-0-7918-3863-1
  • Copyright © 2009 by ASME

abstract

The precise application of proteins and other biomolecules to create patterned surfaces is an important step in many processes, including the creation of biosensors and directed cell growth for tissue engineering [1, 2]. While traditional poly-dimethylsiloxane (PDMS) elastomeric stamps have been used to successfully transfer proteins with sub-micron resolution, the resulting patterns are limited to a single, uniform protein concentration. The technique presented here utilizes varied-topography hydrophilic stamps as a diffusion medium, allowing protein gradients to be easily applied and accurately reproduced. Specifically, stamps of a 2% agarose hydrogel are used to demonstrate variable-concentration patterning of a fluorescently-labeled protein.

Copyright © 2009 by ASME

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