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Single Cell Stimulation Assay: Microfluidic Substance Delivery to a Laterally Trapped Cell

[+] Author Affiliations
Kyohei Terao, Takaaki Suzuki, Fumikazu Oohira

Kagawa University, Takamatsu, Japan

Murat Gel, Masao Washizu

The University of Tokyo, Tokyo, Japan

Atsuhito Okonogi, Hidetoshi Kotera

Kyoto University, Kyoto, Japan

Paper No. AJK2011-36009, pp. 317-319; 3 pages
doi:10.1115/AJK2011-36009
From:
  • ASME-JSME-KSME 2011 Joint Fluids Engineering Conference
  • ASME-JSME-KSME 2011 Joint Fluids Engineering Conference: Volume 2, Fora
  • Hamamatsu, Japan, July 24–29, 2011
  • Conference Sponsors: Fluids Engineering Division
  • ISBN: 978-0-7918-4441-0
  • Copyright © 2011 by JSME

abstract

We propose a novel cell stimulation device for the analysis of cell responses to chemical stimuli. In order to deliver chemical substances to target single cells, we developed a microfluidic device having microchannels and apertures in the side wall to subject stimuli to laterally trapped cells. The channels were designed to allow simple flow control with single syringe pump. We demonstrated single cell trapping and culturing of pancreatic β cell with the device. To test its feasibility in cell stimulation assay, intracellular response of the cell to glucose stimulation was demonstrated.

Copyright © 2011 by JSME
Topics: Microfluidics

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