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Detection of Non-Diffusion-Limited Enzymatic Surface Reaction in Nanofluidic Channels

[+] Author Affiliations
Chuanhua Duan, Dong-Kwon Kim, Arun Majumdar

University of California, Berkeley, Berkeley, CA

Yu-Feng Chen

Industrial Technology Research Institute, Chutung, Taiwan

Paper No. ICNMM2009-82188, pp. 937-941; 5 pages
doi:10.1115/ICNMM2009-82188
From:
  • ASME 2009 7th International Conference on Nanochannels, Microchannels, and Minichannels
  • ASME 2009 7th International Conference on Nanochannels, Microchannels and Minichannels
  • Pohang, South Korea, June 22–24, 2009
  • Conference Sponsors: Nanotechnology Institute
  • ISBN: 978-0-7918-4349-9 | eISBN: 978-0-7918-3850-1
  • Copyright © 2009 by ASME

abstract

Using nanofluidic channels to detect enzymatic surface reactions can overcome diffusion-limited patterning as enzyme accelerates surface reactions without being consumed. In this paper, Trypsin proteolysis reaction is used to demonstrate this idea. Trypsin (enzyme) cleaves Poly-L-Lysine (PLL) coated on the surface of silica nanochannels, resulting in a change of surface charge density and channel height. This change is detected by monitoring the electrical conductance along the nanochannels. 50 μg/ml Trypsin has been detected in 90 nm-height nanochannels within one hour, which is 30 times faster compared to that of a diffusion-limited surface binding reaction. The effect of nanochannel height and the detection limitation are also discussed. Our results indicate that nanofluidic channels can be used for detecting enzymatic-based surface reactions.

Copyright © 2009 by ASME

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