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Microscale Oil-Covered Cell Array (MOCCA): A Droplet Array for High-Content Single-Cell Analysis and Imaging

[+] Author Affiliations
Liang-I Lin, Shih-Hui Chao, Deirdre R. Meldrum

Arizona State University, Tempe, AZ

Paper No. ICNMM2009-82283, pp. 669-672; 4 pages
  • ASME 2009 7th International Conference on Nanochannels, Microchannels, and Minichannels
  • ASME 2009 7th International Conference on Nanochannels, Microchannels and Minichannels
  • Pohang, South Korea, June 22–24, 2009
  • Conference Sponsors: Nanotechnology Institute
  • ISBN: 978-0-7918-4349-9 | eISBN: 978-0-7918-3850-1
  • Copyright © 2009 by ASME


A simple, low-cost technique for high throughput single-cell analysis, Microscale Oil-Covered Cell Array (MOCCA), is presented in this paper. Corresponding to recent research on single cell analysis, simple devices for isolated cell chambers are urgently needed and long sought-after. Instead of using microfabricated solid structures to capture cells, MOCCA isolates cells in discrete aqueous droplets that are separated by oil on the patterned hydrophilic areas on a relatively more hydrophobic flat substrate. In our pioneer study, we created an array of 700-picoliter droplets. The randomly seeded E. coli cell number in each discrete droplet approaches single-cell levels. The total time needed for MOCCA fabrication was no more than 10 minutes. Compared to traditional micro-fabrication techniques, MOCCA dramatically lowers the cost and enhances the efficiency for the fabrication procedure, while producing a microscale array as in those made using traditional methods.

Copyright © 2009 by ASME



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