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Laser-Induced Fluorescence Detection of Copper Ion With a Rhodamine Derivative by a Capillary Electrophoresis Microchip

[+] Author Affiliations
Yong Yu, Feng Shen

Chinese Academy of Science, Beijing, China

Meng Yang

Chinese Academy of Science; Beijing Normal University, Beijing, China

Paper No. ICNMM2009-82071, pp. 665-667; 3 pages
  • ASME 2009 7th International Conference on Nanochannels, Microchannels, and Minichannels
  • ASME 2009 7th International Conference on Nanochannels, Microchannels and Minichannels
  • Pohang, South Korea, June 22–24, 2009
  • Conference Sponsors: Nanotechnology Institute
  • ISBN: 978-0-7918-4349-9 | eISBN: 978-0-7918-3850-1
  • Copyright © 2009 by ASME


A capillary electrophoresis microchip system with a confocal LIF detector was successfully constructed. RBPhOH, a new fluorescence dye synthesized from rhodamine B, was first investigated in a glass microchip for the selective and sensitive determination of copper ion by LIF method. The influences of the pH value, concentration of running buffer, detection voltage, and sample loading time on the response of LIF detector were studied. RBPhOH dissolved in 20 mM pH 6.0 Tris containing 10% acetonitrile could detect copper ions with a detection limit of 1.34×10− 7 mol L−1 (S/N=3), an order of magnitude lower than those of conductivity detectors.

Copyright © 2009 by ASME



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