0

Full Content is available to subscribers

Subscribe/Learn More  >

Microfluidic Devices for Rapid Protein Separation

[+] Author Affiliations
Z. Hugh Fan, Champak Das, Cesar Moreira, Daniel Olivero, Hong Chen

University of Florida, Gainesville, FL

Paper No. MicroNano2008-70208, pp. 513-516; 4 pages
doi:10.1115/MicroNano2008-70208
From:
  • 2008 Second International Conference on Integration and Commercialization of Micro and Nanosystems
  • 2008 Second International Conference on Integration and Commercialization of Micro and Nanosystems
  • Clear Water Bay, Kowloon, Hong Kong, June 3–5, 2008
  • Conference Sponsors: Nanotechnology Institute
  • ISBN: 0-7918-4294-0 | eISBN: 0-7918-3819-6
  • Copyright © 2008 by ASME

abstract

We present our investigation of using microfluidic devices for rapid protein separation. The devices were made from cyclic olefin copolymers that have high optical clarity and high glass transition temperature. Protein separation was achieved by using isoelectric focusing (IEF) and polyacrylamide gel electrophoresis (PAGE). A laser-induced fluorescence (LIF) imaging system was developed to detect proteins while they migrated under an electric field. IEF was carried out in a separation medium consisting of carrier ampholytes and a mixture of linear polymers. Dynamic coating of the linear polymers prevented proteins from adsorption and suppressed electroosmotic flows. PAGE was achieved in twenty-nine parallel channels. In addition, we integrated IEF with PAGE in a microfluidic device for two-dimensional protein separation.

Copyright © 2008 by ASME

Figures

Tables

Interactive Graphics

Video

Country-Specific Mortality and Growth Failure in Infancy and Yound Children and Association With Material Stature

Use interactive graphics and maps to view and sort country-specific infant and early dhildhood mortality and growth failure data and their association with maternal

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging and repositioning the boxes below.

Related eBook Content
Topic Collections

Sorry! You do not have access to this content. For assistance or to subscribe, please contact us:

  • TELEPHONE: 1-800-843-2763 (Toll-free in the USA)
  • EMAIL: asmedigitalcollection@asme.org
Sign In