0

Full Content is available to subscribers

Subscribe/Learn More  >

Bead-Based Miniature Microfluidic Systems for Rapid RNA Extraction and Reverse Transcription

[+] Author Affiliations
Kang-Yi Lien, Chien-Ju Liu, Jyh-Wei Shin, Tsuey-Yu Chang, Gwo-Bin Lee

National Cheng Kung University, Tainan, Taiwan

Paper No. MNHT2008-52268, pp. 251-256; 6 pages
doi:10.1115/MNHT2008-52268
From:
  • ASME 2008 First International Conference on Micro/Nanoscale Heat Transfer
  • ASME 2008 First International Conference on Micro/Nanoscale Heat Transfer, Parts A and B
  • Tainan, Taiwan, June 6–9, 2008
  • Conference Sponsors: Nanotechnology Institute
  • ISBN: 0-7918-4292-4 | eISBN: 0-7918-3813-7
  • Copyright © 2008 by ASME

abstract

The current study presents a new integrated microfluidic chip for rapid ribonucleic acid (RNA) purification, extraction and reverse transcription (RT) in an automatic fashion. The miniature system consists of two individual functional devices including a two-way microfluidic control module and a magnetic field/temperature control module. The functional microfluidic control module can perform pumping, mixing, purification and concentration of the RNA samples by incorporating with the magnetic bio-separator consisting of 2-dimension twisted microcoils. Notably, the magnetic bio-separators are developed either to perform the separation of magnetic beads or to control the temperature field for the subsequent RT process. Experimental results showed that the total RNA was successfully purified and extracted by the magnetic beads, and the subsequent RT process of RNA was completed automatically. As a whole, the developed system may provide a powerful platform for biomedical application and biological analysis.

Copyright © 2008 by ASME
Topics: Microfluidics , RNA

Figures

Tables

Interactive Graphics

Video

Country-Specific Mortality and Growth Failure in Infancy and Yound Children and Association With Material Stature

Use interactive graphics and maps to view and sort country-specific infant and early dhildhood mortality and growth failure data and their association with maternal

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging and repositioning the boxes below.

Related eBook Content
Topic Collections

Sorry! You do not have access to this content. For assistance or to subscribe, please contact us:

  • TELEPHONE: 1-800-843-2763 (Toll-free in the USA)
  • EMAIL: asmedigitalcollection@asme.org
Sign In