0

Full Content is available to subscribers

Subscribe/Learn More  >

Application of an Optically Induced Electrokinetic Manipulation Technique on Live Bacteria

[+] Author Affiliations
Jae-Sung Kwon, Sandeep Ravindranath, Aloke Kumar, Joseph Irudayaraj, Steven T. Wereley

Purdue University, West Lafayette, IN

Paper No. IMECE2010-39324, pp. 815-821; 7 pages
doi:10.1115/IMECE2010-39324
From:
  • ASME 2010 International Mechanical Engineering Congress and Exposition
  • Volume 10: Micro and Nano Systems
  • Vancouver, British Columbia, Canada, November 12–18, 2010
  • Conference Sponsors: ASME
  • ISBN: 978-0-7918-4447-2
  • Copyright © 2010 by ASME

abstract

In this paper, we apply a recently demonstrated rapid electrokinetic patterning (REP) technique to Shewanella oneidensis MR-1 and confirm the application possibility of the technique on bio-materials. This technique utilizes a simple microfluidic chip with two indium tin oxide (ITO) coated parallel electrodes and requires the simultaneous application of AC electric field and the hologram provided from an infrared (1064 nm) laser, in order to control and aggregate particles onto electrodes. For the experimentation in this paper, Shewanella oneidensis MR-1 are cultured by green fluorescent protein (GFP) on Luria-Bertani (LB) agar plate and through sample pretreatment process, mid-log phase samples of the bacteria finally are obtained. As a result of applying REP technique to this bacteria sample, we could collect a lot of the bacteria onto ITO electrode surfaces rapidly and the bacteria cluster could be translated with the movement of laser focus position under uniform electric field. Also it was confirmed that there exists a particular frequency range, i.e. critical frequency for REP based bacteria cluster. We could find that under the application of REP technique, the ellipsoidal bacteria are reoriented with respect to electric field lines as electrical frequency increases up. Also the change in the vertical distance between the bacteria and the electrode surface with electrical voltages could be observed. These results show that the REP technique can be used to separate, sort and manipulate bioparticles like bacteria.

Copyright © 2010 by ASME
Topics: Bacteria

Figures

Tables

Interactive Graphics

Video

Country-Specific Mortality and Growth Failure in Infancy and Yound Children and Association With Material Stature

Use interactive graphics and maps to view and sort country-specific infant and early dhildhood mortality and growth failure data and their association with maternal

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging and repositioning the boxes below.

Related eBook Content
Topic Collections

Sorry! You do not have access to this content. For assistance or to subscribe, please contact us:

  • TELEPHONE: 1-800-843-2763 (Toll-free in the USA)
  • EMAIL: asmedigitalcollection@asme.org
Sign In