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Lysing E. Coli Using Titanium Dioxide Particles for Lab-on-a-Chip Applications

[+] Author Affiliations
Weijie Wan, John T. W. Yeow

University of Waterloo, Waterloo, ON, Canada

Paper No. IMECE2010-38626, pp. 749-753; 5 pages
doi:10.1115/IMECE2010-38626
From:
  • ASME 2010 International Mechanical Engineering Congress and Exposition
  • Volume 10: Micro and Nano Systems
  • Vancouver, British Columbia, Canada, November 12–18, 2010
  • Conference Sponsors: ASME
  • ISBN: 978-0-7918-4447-2
  • Copyright © 2010 by ASME

abstract

A novel method is proposed and demonstrated to be able to lyse E. Coli bacteria cells for Lab-on-a-Chip applications. The proposed method uses titanium dioxide particles as a photocatalyst and a miniaturized UV LED array as an excitation light source on the microchip level to perform cell lysis. The experimental result demonstrates the feasibility of the proposed novel device. The working device suggests an inexpensive, easy to be fabricated and effective way for microchip cell lysis. The miniaturized UV LED array and the microchip with a reaction chamber can be easily integrated with other functional components to form a customized whole Lab-on-a-Chip system.

Copyright © 2010 by ASME

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