0

Full Content is available to subscribers

Subscribe/Learn More  >

Quantifying the Nanomechanical Properties of Bacillus Anthracis and Implications for Spore Killing

[+] Author Affiliations
Terri A. Camesano, Paola A. Pinzón-Arango

Worcester Polytechnic Institute, Worcester, MA

Ramanathan Nagarajan

Natick Soldier Research, Development and Engineering Center, Natick, MA

Paper No. NEMB2010-13032, pp. 331-332; 2 pages
doi:10.1115/NEMB2010-13032
From:
  • ASME 2010 First Global Congress on NanoEngineering for Medicine and Biology
  • ASME 2010 First Global Congress on NanoEngineering for Medicine and Biology
  • Houston, Texas, USA, February 7–10, 2010
  • Conference Sponsors: ASME Nanotechnology Council
  • ISBN: 978-0-7918-4392-5 | eISBN: 978-0-7918-3866-2
  • Copyright © 2010 by ASME

abstract

Atomic force microscopy (AFM) was used to study the changes in the nanomechanical properties of the surface of Bacillus anthracis spores during germination. The Hertz model of continuum mechanics of contact was used to evaluate the Young’s or tensile elastic modulus of the spores before and after germination by applying the model to load-indentation curves obtained during force cycles. The highest elastic modulus was obtained with dormant spores, with average elasticity values of 197 ± 81 MPa. Fully vegetative spores had the lowest elasticity values. The elasticity decreased when spores were incubated with either L-alanine or inosine, and the decrease was greatest when both of these germinants were used in combination. We also found that as the spore elasticity values increasd, the indentation depth of the AFM probe into the surface increased, with vegetative B. anthracis cells having elasticity depths of up to 246.2 nm. We have shown that spore nanomechanical properties change during germination, and depend on the type of germinant that is used. Weakening of the spore cell wall may help explain why germinating cells are much more susceptible to anti-sporal agents. The study of elasticity of spores may be a valuable tool in the development of antibiotics or anti-sporal treatments.

Copyright © 2010 by ASME

Figures

Tables

Interactive Graphics

Video

Country-Specific Mortality and Growth Failure in Infancy and Yound Children and Association With Material Stature

Use interactive graphics and maps to view and sort country-specific infant and early dhildhood mortality and growth failure data and their association with maternal

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging and repositioning the boxes below.

Related eBook Content
Topic Collections

Sorry! You do not have access to this content. For assistance or to subscribe, please contact us:

  • TELEPHONE: 1-800-843-2763 (Toll-free in the USA)
  • EMAIL: asmedigitalcollection@asme.org
Sign In