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Effects of an Experimental Drug, Suberoylanilide Hydroxamic Acid (SAHA), on Attachment, Spreading, and Stiffness of Human Breast Cancer Cells on Silicon Substrates

[+] Author Affiliations
Jeannine S. Strobl, Mehdi Nikkhah, Robert Rhoades, Masoud Agah

Virginia Tech; Edward Via Virginia College of Osteopathic Medicine, Blacksburg, VA

Paper No. NEMB2010-13037, pp. 161-162; 2 pages
doi:10.1115/NEMB2010-13037
From:
  • ASME 2010 First Global Congress on NanoEngineering for Medicine and Biology
  • ASME 2010 First Global Congress on NanoEngineering for Medicine and Biology
  • Houston, Texas, USA, February 7–10, 2010
  • Conference Sponsors: ASME Nanotechnology Council
  • ISBN: 978-0-7918-4392-5 | eISBN: 978-0-7918-3866-2
  • Copyright © 2010 by ASME

abstract

We are investigating silicon-based platforms for detection and analysis of breast cancer cells. Attachment and spreading of MDA-MB-231 human metastatic breast cancer cells was compared to that of non-tumorigenic human breast epithelial cells, MCF-10A, and the impact of SAHA (Vorinostat), a histone deacetylase (HDAC) inhibitor, on cell behaviors was ascertained. Our results showed the cancer cells attached to flat silicon and PECVD nitride-coated silicon more efficiently than non-cancer cells, and preferential cancer cell attachment was enhanced by SAHA. Fluorescent immunohistochemistry (IHC) revealed that SAHA stimulated actin stress fiber formation and focal adhesion to the substrates; atomic force microscopy (AFM) showed SAHA increased the cancer cell stiffness. Collectively, SAHA-induced biomechanical changes altered the cell morphology and mode of attachment to flat silicon and to three-dimensional silicon microstructures. This is the first report of the use of AFM to characterize the biomechanical effects of a HDAC inhibitor in cancer cells.

Copyright © 2010 by ASME
Topics: Cancer , Drugs , Silicon , Stiffness

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