0

Full Content is available to subscribers

Subscribe/Learn More  >

Nanochannels Fabrication, Filling and DNA Manipulation

[+] Author Affiliations
V. R. S. S. Mokkapati, J. Mollinger, J. Bastemeijer, A. Bossche

Delft University of Technology, Delft, The Netherlands

V. Di Virgilio

Universitat Politecnica de Catalunya, Barcelona, Spain

Paper No. NEMB2010-13281, pp. 135-138; 4 pages
doi:10.1115/NEMB2010-13281
From:
  • ASME 2010 First Global Congress on NanoEngineering for Medicine and Biology
  • ASME 2010 First Global Congress on NanoEngineering for Medicine and Biology
  • Houston, Texas, USA, February 7–10, 2010
  • Conference Sponsors: ASME Nanotechnology Council
  • ISBN: 978-0-7918-4392-5 | eISBN: 978-0-7918-3866-2
  • Copyright © 2010 by ASME

abstract

Fabrication of nanochannels is drawing considerable interest due to its broad applications in nanobiotechnology (e.g. biomolecular sensing and single DNA manipulation). Nanochannels offer distinct advantages in allowing a slower translocation and multiple sensing spots along the channel both of which improve the read-out resolution. However, implementing electrodes inside nanochannel has rarely been demonstrated to our knowledge. Therefore, we are highly motivated to do this research. The device described in this work is a Si-Glass anodically bonded Lab-on-a-Chip (LOC) device of a few millimeters in size using femtoliters in volume capable of performing DNA manipulation. The LOC structure is based on two mainstream microchannels interconnected by nanochannels. Organic samples as DNA will be released in the microfluidic mainstream and then, once confined in the nanochannels, are observed, manipulated and analyzed. We expect the general folded shape of DNA evolves to the unfolded linear shape due to the confinement in narrow channels. This shape is especially suitable for advanced manipulation and analysis.

Copyright © 2010 by ASME
Topics: Manufacturing , DNA

Figures

Tables

Interactive Graphics

Video

Country-Specific Mortality and Growth Failure in Infancy and Yound Children and Association With Material Stature

Use interactive graphics and maps to view and sort country-specific infant and early dhildhood mortality and growth failure data and their association with maternal

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging and repositioning the boxes below.

Related eBook Content
Topic Collections

Sorry! You do not have access to this content. For assistance or to subscribe, please contact us:

  • TELEPHONE: 1-800-843-2763 (Toll-free in the USA)
  • EMAIL: asmedigitalcollection@asme.org
Sign In