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Continuous-Flow Microfluidic Devices for Detection of Bacterial Endospores

[+] Author Affiliations
Jiandi Wan, Pavel Landsman, Bing Xia, Paul E. Bower, Volkmar Heinrich, Guilford Jones, II, Valentine I. Vullev

Boston University, Boston, MA

Paper No. NANOBIO2006-18030, pp. 23-24; 2 pages
doi:10.1115/NANOBIO2006-18030
From:
  • ASME 2006 Frontiers in Biomedical Devices Conference
  • ASME 2006 Frontiers in Biomedical Devices Conference
  • Irvine, California, USA, June 8 – 9, 2006
  • Conference Sponsors: Nanotechnology Institute
  • ISBN: 0-7918-4763-2 | eISBN: 0-7918-3781-5
  • Copyright © 2006 by ASME

abstract

Using non-lithographic fabrication methods developed in our laboratory, we prepared continuous-flow microfluidic analyzers. We tested these devices in experiments involving detection of bacterial endospores. The detection was based on the enhancement of the fluorescence of a cationic dye, 3,3′-diethylthiacyanine iodide (THIA), in the presence of spores. We were able to detect as few as ~105 spores when injected in a device. The measurements with the micro-fluidic devices manifested significantly higher sensitivity for bacterial spores than for vegetative bacteria. Such distinction between spores and vegetative bacteria could not be achieved with THIA using steady-state emission measurements.

Copyright © 2006 by ASME

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