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Characterization of the Effect of Geometry on Single Cell Adhesion Strength Using a Microfluidic Device

[+] Author Affiliations
Kevin V. Christ, Kyle B. Williamson, Kristyn S. Masters, Kevin T. Turner

University of Wisconsin at Madison, Madison, WI

Paper No. IMECE2007-43775, pp. 809-810; 2 pages
doi:10.1115/IMECE2007-43775
From:
  • ASME 2007 International Mechanical Engineering Congress and Exposition
  • Volume 10: Mechanics of Solids and Structures, Parts A and B
  • Seattle, Washington, USA, November 11–15, 2007
  • Conference Sponsors: ASME
  • ISBN: 0-7918-4304-1 | eISBN: 0-7918-3812-9
  • Copyright © 2007 by ASME

abstract

Cell adhesion plays a crucial role in a number of fundamental physiological processes and is important in the development of implantable biomaterials. Cell adhesion strength has previously been measured using a range of techniques, including population assays (e.g., centrifugation [1], hydrodynamic flow [2]) and single-cell methods (e.g., AFM [3], micropipette manipulation [4]). Population studies are unable to provide detailed information about individual cell behavior, while the single-cell methods are often time-consuming and difficult to perform. Microfluidic channels present a way to generate well-defined stress fields on cells [5]. The small dimensions of these channels result in low Reynolds numbers that allow for the generation of sufficiently large stresses to detach well-spread cells under laminar flow conditions. In the present work, a microfluidic channel was used to controllably load adhered single-cells to detachment and measure the adhesion strength. Using this assay, the effect of cell geometry on adhesion strength was investigated.

Copyright © 2007 by ASME

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